Select the image of interest and switch to the Edit tab. Scroll down to the annotation and click the Edit button to open the interactive annotation editor.

You can clear away any existing annotations by using Tools > Clear

For E-Test strips, the polygon tool is highly useful. Select the tool and outline the 3 first corners by clicking with your mouse. For the final corner double-click to confirm the outline.

If you are done, click the Save annotation button

The updated annotation should now be visible in the preview and sidebar.

For the diskImageR-style analysis a ZOI shape needs to be provided. While we included an automated algorithm we have observed that it may be more efficient to apply the annotation manually.

Click the Edit button for the ZOI shape annotation.

Use the pen tool with a large brush size to roughly outline the ZOI

Interiors can be filled using the Fill tool.

J-AST provides a special symmetry tool that allows to symmetrize the image according to a drawn symmetry axis.

For E-Test strips, dragging the axis in the middle of the strip will produce a suitable mask

Use the Save annotation button to confirm the changes

Use the interactive editing tools to

• fix mistakes with the strip segmentation

• provide a ZOI shape for the first time point

Note: The ZOI shape only needs to be present for the first time point!

Then use the Copy and register ZOI shape across timeline tool to automatically copy the ZOI shape from the first time point to all others. The algorithm uses the strip as reference and will handle image rotation automatically.

The images now have all areas annotated.