To find the MIC, select all E-Tests which can be done by deselect all images and then clicking the Select all E-Tests button.

The MIC analysis requires that all images have a plate annotation, strip annotation, pixel size, and a E-Test strip preset.

To set the preset, click the Set E-Test strip preset button

J-AST provides two presets (E 256-0.016 and E 32-0.002). Additional presets can be added through the Presets button at the top of the page.

All images use the E256 preset, so apply the configuration as shown in the picture.

Confirm that the preset is set which is indicated by a badge next to the image preview

Start the MIC detection workflow by navigating to Process > Analyze > Auto-detect MIC v1

Here you can set a variety of parameters if required.

Start the run by clicking OK.

After the run has finished the results can be found in the Results page.

Note: If you enabled Set MIC metadata in the analysis settings, the MIC will be also written into the image and can be reviewed through the Edit page.

The results are stored in the mic_results.csv file

The last table column contains the MIC.

Please note that the success of the algorithm depends on the properties of the input data. A clear ZOI boundary is preferable. The pipeline detects growth inside the ZOI and may reject the image unless Enable N/A detection is turned off.