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Contribution of Radixin and Ezrin to the maintenance of hepatocytes’ excretory function in health and disease.

Dellbrügge F, Jesse LD, Medyukhina A, Liu N, Neugebauer S, Freißmuth M, Höppener S, Figge MT, Morrison H, Riecken LB, Press AT#

Background & aimsExcretory liver failure is frequently associated with poor prognosis in critically ill patients. It is characterized by the loss of canalicular membrane export pumps at the hepatocyte membrane. The membrane export pump Multidrug resistant-associated protein (MRP) 2 is pivotal in hepatocytes for brushed membrane morphology and transport of various metabolites. In addition, MRP2 […]

Hydrophilic cryogels as potential 3D cell culture materials: Synthesis and characterization of 2-(methacrylamido) glucopyranose-based polymer scaffolds.

Behrendt F, Pretzel D, Cseresnyés Z, Kleinsteuber M, Wloka T, Radosa L, Figge MT, Gottschaldt M, Brakhage AA, Schubert US#

Cell cultures are important techniques to investigate cellular processes in a simplified manner. The most commonly applied 2D models do not simulate most of the in vivo conditions whereas cryogels as promising 3D cell culture materials are able to provide both a structured microenvironment and tailored surface properties, similar to the natural extracellular matrix (ECM). […]

The role of pneumococcal extracellular vesicles on the pathophysiology of the kidney disease hemolytic uremic syndrome.

Battista M, Hoffmann B, Bachelot Y, Zimmermann L, Teuber L, Jost A, Linde S, Westermann M, Müller MM, Slevogt H, Hammerschmidt S, Figge MT, Vilhena C*#, Zipfel PF*#

Streptococcus pneumoniae-induced hemolytic uremic syndrome (Sp-HUS) is a kidney disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, and acute kidney injury. This disease is frequently underdiagnosed and its pathophysiology is poorly understood. In this work, we compared clinical strains, isolated from infant Sp-HUS patients, with a reference pathogenic strain D39, for host cytotoxicity and further explored […]

Evaluation of reproducible cryogel preparation based on automated image analysis using deep learning.

Behrendt F*, Cseresnyés Z*, Gerst R, Gottschaldt M, Figge MT#, Schubert US#

Cryogels represent a class of porous sponge-like materials possessing unique properties including high-fidelity reproduction of tissue structure and maximized permeability. Their architecture is mainly based on an interconnected network of macropores that provides sufficient stability whilst allowing the movement of substances through the material. In most cryogel applications, the pore size is very important, especially […]

Aspergillus fumigatus hijacks human p11 to redirect fungal-containing phagosomes to non-degradative pathway.

Jia LJ, Rafiq M, Radosa L, Hortschansky P, Cunha C, Cseresnyés Z, Krüger T, Schmidt F, Heinekamp T, Straßburger M, Loeffler B, Doenst T, Lacerda JF, Campos A, Figge MT, Carvalho A, Kniemeyer O, Brakhage AA

The decision whether endosomes enter the degradative or recycling pathway in mammalian cells is of fundamental importance for pathogen killing, and its malfunctioning has pathological consequences. We discovered that human p11 is a critical factor for this decision. The HscA protein present on the conidial surface of the human-pathogenic fungus Aspergillus fumigatus anchors p11 on […]

The lipid raft-associated protein stomatin is required for accumulation of dectin-1 in the phagosomal membrane and for full activity of macrophages against Aspergillus fumigatus.

Goldmann M, Schmidt F, Cseresnyés Z, Orasch T, Jahreis S, Hartung S, Figge MT, von Lilienfeld-Toal M, Heinekamp T, Brakhage AA

Alveolar macrophages are among the first cells to come into contact with inhaled fungal conidia of the human pathogenic fungus Aspergillus fumigatus. In lung alveoli, they contribute to phagocytosis and elimination of conidia. As a counter defense, conidia contain a grey-green pigment allowing them to survive in phagosomes of macrophages for some time. Previously, we […]

Tuning the corona-core ratio of polyplex micelles for selective oligonucleotide delivery to hepatocytes or hepatic immune cells.

Foo WL, Cseresnyés Z, Rössel C, Teng Y, Ramoji A, Chi M, Hauswald W, Huschke S, Hoeppener S, Popp J, Schacher FH, Sierka M, Figge MT, Press AT#, Bauer M#

Targeted delivery of oligonucleotides or small molecular drugs to hepatocytes, the liver’s parenchymal cells, is challenging without targeting moiety due to the highly efficient mononuclear phagocyte system (MPS) of the liver. The MPS comprises Kupffer cells and specialized sinusoidal endothelial cells, efficiently clearing nanocarriers regardless of their size and surface properties. Physiologically, this non-parenchymal shield […]

JIPipe: Visual batch processing for ImageJ.

Gerst R*, Cseresnyés Z*, Figge MT#

The continuous development of new microscopy techniques requires the parallel evolution of image analysis workflows. ImageJ provides a high level of accessibility to bioimage processing, which is still impeded by the necessity of developing scripts to achieve reproducibility, and to comply to the FAIR principles. We provide a visual language termed JIPipe that allows the […]

Targeting of phagolysosomes containing conidia of the human pathogenic fungus Aspergillus fumigatus with polymeric particles.

González K*, Gangapurwala G*, Alex J*, Vollrath A, Cseresnyés Z, Weber C, Czaplewska JA, Hoeppener S, Svensson CM, Orasch T, Heinekamp T, Guerrero-Sánchez C, Figge MT, Schubert US, Brakhage AA

Conidia of the airborne human-pathogenic fungus Aspergillus fumigatus are inhaled by humans. In the lung, they are phagocytosed by alveolar macrophages and intracellularly processed. In macrophages, however, conidia can interfere with the maturation of phagolysosomes to avoid their elimination. To investigate whether polymeric particles (PPs) can reach this intracellular pathogen in macrophages, we formulated dye-labeled […]

Analysis of HDACi-coupled Nanoparticles: Opportunities and challenges.

Kühne M, Hofmann S, Lindemann H, Cseresnyés Z, Dzierza A, Schröder D, Godmann M, Koschella A, Eggeling C, Fischer D, Figge MT, Heinze T, Heinzel T

Systemic administration of histone deacetylase inhibitors (HDACi), like valproic acid (VPA), is often associated with rapid drug metabolization and untargeted tissue distribution. This requires high-dose application that can lead to unintended side effects. Hence, drug carrier systems such as nanoparticles (NPs) are developed to circumvent these disadvantages by enhancing serum half-life as well as organ […]