In this project, the growth of fungal hyphae is modeled. As a parameterized approach, different hyphal structures can be learned, simulated and reproduced for further analysis or applications. The algorithm is implemented as a recursive approach, which allows to potentially grow a new hyphal branch at each depth level. The angle of curvature, frequency of new branching, and piercing (in the alveolus) is also parameterized, making it highly adaptable for different scenarios. Interactions with immune cells (alveolar macrophages) is realized according to the set of rules within the agent-based modeling.
Hyphal growth simulation
Publications
Evaluation of reproducible cryogel preparation based on automated image analysis using deep learning.
Behrendt F*, Cseresnyés Z*, Gerst R, Gottschaldt M, Figge MT#, Schubert US#
Cryogels represent a class of porous sponge-like materials possessing unique properties including high-fidelity reproduction of tissue structure and maximized permeability. Their architecture is mainly based on an interconnected network of macropores that provides sufficient stability whilst allowing the movement of substances through the material. In most cryogel applications, the pore size is very important, especially […]
Aspergillus fumigatus hijacks human p11 to redirect fungal-containing phagosomes to non-degradative pathway.
Jia LJ, Rafiq M, Radosa L, Hortschansky P, Cunha C, Cseresnyés Z, Krüger T, Schmidt F, Heinekamp T, Straßburger M, Loeffler B, Doenst T, Lacerda JF, Campos A, Figge MT, Carvalho A, Kniemeyer O, Brakhage AA
The decision whether endosomes enter the degradative or recycling pathway in mammalian cells is of fundamental importance for pathogen killing, and its malfunctioning has pathological consequences. We discovered that human p11 is a critical factor for this decision. The HscA protein present on the conidial surface of the human-pathogenic fungus Aspergillus fumigatus anchors p11 on […]
The lipid raft-associated protein stomatin is required for accumulation of dectin-1 in the phagosomal membrane and for full activity of macrophages against Aspergillus fumigatus.
Goldmann M, Schmidt F, Cseresnyés Z, Orasch T, Jahreis S, Hartung S, Figge MT, von Lilienfeld-Toal M, Heinekamp T, Brakhage AA
Alveolar macrophages are among the first cells to come into contact with inhaled fungal conidia of the human pathogenic fungus Aspergillus fumigatus. In lung alveoli, they contribute to phagocytosis and elimination of conidia. As a counter defense, conidia contain a grey-green pigment allowing them to survive in phagosomes of macrophages for some time. Previously, we […]