Confrontation assay model

In this study we derived a framework to analyze confrontation assays by an image based systems biology approach.

Experimental Data

Confrontations assays were performed with Candida glabrata and neutrophils. Time-resolved microscopy data were collected during the whole duration of the experiment.

Experimental collaborators

Fungal Septomics group at the Leibniz-HKI in Jena, Germany

Image Analysis

Experimental data sets were quantified and analyzed with the aid of automated image analysis using the tracking tool AMIT.

Model

We developed a stochastic state-based model that describes the dynamics of the confrontation assay. The simulated kinetics are fitted to the experimental data so that the underlying model parameters of the system can be determined.

Image-based modelling of confrontation assays

Live cell imaging data is analyzed and quantified using AMIT. Based on these quantifications the state-based model for confrontation assays is calibrated and model parameters can be estimated.

Publications

NFDI4BIOIMAGE – National research data infrastructure for microscopy and bioimage analysis

Moore J#, Kunis S, Grüning B, Blank-Burian M, Mallm JP, Stöter T, Zuschratter W, Figge MT, Kreshuk A, Tischer C, Haase R, Zobel T, Bauer P, Svensson CM, Gerst R, Hanne J, Schmidt C, Becker MM, Bocklitz T, Bumberger J, Chalopin C, Chen J, Czodrowski P, Dickscheid T, Fortmann- Grote C, Huisken J, Lohmann J, Schauss A, Baumann M, Beretta C, Burel JM, Heuveline C, Kuner R, Kuner T, Landwehr M, Leibfried A, Nitschke R, Mittal D, von Suchodoletz D, Valencia- Schneider M, Zentis P, Brilhaus D, Hartley M, Hülsmann B, Dunker S, Keppler A, Mathur A, Meesters C, Möbius W, Nahnsen S, Pfander C, Rehwald S, Serrano-Solano B, Vilardell Scholten L, Vogl R, Becks L, Ferrando-May E*#, Weidtkamp-Peters S*#

August 7, 2024

Bioimaging refers to a collection of methods to visualize the internal structures and mechanisms of living organisms. The fundamental tool, the microscope, has enabled seminal discoveries like that of the cell as the smallest unit of life, and continues to expand our understanding of biological processes. Today, we can follow the interaction of single molecules […]

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Unique target binding by the C-terminal Region of FHR1 provides a new perception of aHUS Pathology

July 23, 2024

FHR1 is a multifunctional human plasma protein with three C-terminal domains, namely short consensus repeats (SCR) 3-5, showing 98% sequence-identity with the complement inhibitor Factor H. We show that FHR1 uses all three C-terminal SCR to make surface contact. The conserved C-terminal regions of FHR1 and Factor H are altered in patients with atypicalhemolytic-uremic-syndrome. Therefore, […]

Competitive inhibition and mutualistic growth in Co infections: deciphering Staphylococcus aureus – Acinetobacter baumannii interaction dynamics

Timme S*, Wendler S*, Klassert TE, Saraiva JP, Nunes da Rocha U, Wittchen M, Schramm S, Ehricht R, Monecke S, Edel B, Rödel J, Löffler B, Soledad Ramirez M, Slevogt H, Figge MT*#, Tuchscherr L*#

June 10, 2024

Staphylococcus aureus (Sa) and Acinetobacter baumannii (Ab) are frequently co-isolated from polymicrobial infections that are severe and refractory to therapy. Here, we apply a combination of wet-lab experiments and in silico modeling to unveil the intricate nature of the Ab/Sa interaction using both, representative laboratory strains and strains co-isolated from clinical samples.This comprehensive methodology allowed […]

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